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Goat Anti-AKR1C3 Antibody (EB06637)

Code Name Applications Availability Size Species reactivity Price Grade  
EB06637 Goat Anti-AKR1C3 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $315.00 Elite

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EB06637 1
EB06637 (0.03µg/ml) staining of human breast lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: type IIb 3-alpha hydroxysteroid dehydrogenase, type II 3a-hydroxysteroid dehydrogenase, dihydrodiol dehydrogenase X, chlordecone reductase, aldo-keto reductase family 1, member C3, DDX, trans-1,2-dihydrobenzene-1,2-diol dehydrogenase, type IIb 3-alpha hydroxysteroid dehydrogenase, hydroxysteroid (17-beta) dehydrogenase 5, chlordecone reductase homolog, dihydrodiol dehydrogenase 3, prostaglandin F synthase, aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II), KIAA0119, hluPGFS, HSD17B5, HA1753, HAKRe, HAKRB, DD3, AKR1C3
Official Symbol: AKR1C3
Accession Number(s): NP_003730.4
Human GeneID(s): 8644

Immunogen

Peptide with sequence CFASHPNYPYSDEY, from the C Terminus of the protein sequence according to NP_003730.4.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 35kDa band observed in human liver and human breast lysates (calculated MW of 36.9kDa according to NP_003730). Recommended concentration: 0.01-0.1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Specific Reference

The goat polyclonal antibody used in this paper was manufactured by us:
Gavelová M, Hladíková J, Vildová L, Novotná R, Vondrácek J, Krcmár P, Machala M, Skálová L.
Reduction of doxorubicin and oracin and induction of carbonyl reductase in human breast carcinoma MCF-7 cells.
Chem Biol Interact. 2008 Oct 22;176(1):9-18.
PMID: 18755171

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