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Goat Anti-AKT1 Antibody (EB06875)

Code Name Applications Availability Size Tested species Price Grade  
EB06875 Goat Anti-AKT1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $315.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB06875 1
EB06875 (2µg/ml) staining of MCF7 (A) and NIH3T3 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence
Click here to download PDF datasheet.

Target Protein

Principal Names: AKT1, v-akt murine thymoma viral oncogene homolog 1, HGNC:391, MGC99656, PKB, PRKBA, RAC, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, murine thymoma viral (v-akt) oncogene homolog-1, protein kinase B, rac protein kinase alpha
Official Symbol: AKT1
Accession Number(s): NP_005154.2; NP_001014432.1; NP_001014431.1
Human GeneID(s): 207
Important Comments: Variants one, two and three encode the same protein (NP_005154, NP_001014432, NP_001014431). No cross-reactivity expected with AKT2 or AKT3.

Immunogen

Peptide with sequence C-EVSLAKPKHR, from the internal region of the protein sequence according to NP_005154.2; NP_001014432.1; NP_001014431.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 60kDa band observed in MCF7 and approx. 60-65kDa in NIH3T3 cell lysates (calculated MW of 55.7kDa according to Human NP_005154.2 and Mouse NP_033782.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 2-3ug/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Cow

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