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Goat Anti-AKT3 Antibody (EB06562)

Code Name Applications Availability Size Tested species Price Grade  
EB06562 Goat Anti-AKT3 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB06562 1
EB06562 (1µg/ml) staining of HepG2 (A), Jurkat (B), Mouse Brain (C) and Rat Brain (D) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06562 2
HEK293 overexpressing AKT3 (RC224750) and probed with EB06562 (mock transfection in second lane), tested by Origene.
EB06562 3
EB06562 (5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06562 4
EB06562 (5µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: AKT3, PKBG, PRKBG, STK-2, RAC-gamma, RAC-PK-gamma, DKFZP434N0250, v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma), protein kinase B gamma, serine threonine protein kinase, Akt-3, RAC-gamma serine/threonine protein kinase
Official Symbol: AKT3
Accession Number(s): NP_005456.1; NP_859029.1
Human GeneID(s): 10000
Important Comments: This antibody is expected to recognise an epitope corresponding to aa 119-133 of both isoforms of human AKT3 protein. This antibody does not cross-react with human AKT1/2. Reported variants represent identical protein (NP_859029.1; NP_001193658.1).

Immunogen

Peptide with sequence CSPTSQIDNIGEEEM, from the internal region of the protein sequence according to NP_005456.1; NP_859029.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: A 50-55kDa band observed in lysates of cell lines HepG2 and Jurkat and in Mouse and Rat Brain lysates (calculated MW of 54.0kDa according to NP_859029.1). Recommended concentration: 1-3µg/ml.
IHC: Paraffin embedded Human Prostate and Heart. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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