Change country
Tel: 888-320-GOAT (4628)
Fax: 888-841-9041
Home

Goat Anti-AKT3 Antibody (EB06562)

Related Products: EBP06562 - Immunizing Peptide, Goat Anti-AKT3 Antibody - Trial
Code Name Applications Availability Size Species reactivity Price Grade  
EB06562 Goat Anti-AKT3 Antibody ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog, Cow $300.00 Elite

Ordering Information for

Order direct from Everest

$35.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA.

Order offline

Now shipping direct to US customers! All orders will be shipped to you by our logistics partner in Kentucky. Almost all products are held in stock for immediate shipment, see individual product pages for availability. Orders are placed with and invoiced by Everest Biotech Ltd in the United Kingdom.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (sales@everestbiotech.com), fax (888-841-9041), phone (888-320-GOAT) or mail. Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 09.30 PST / 12.30 EST on Monday through Thursday are shipped the same day.

OR

Order from our local distributor

Axxora, LLC

6181 Cornerstone Court East Suite 103,
San Diego
CA 92121-4727
USA

Tel: 858-550-8830
Toll Free Tel: 1-800-550-3033
Fax: 858-550-8825
Toll Free Fax: 1-800-550-8825
Email: axxora-usa@axxora.com
Website: www.axxora.com
EB06562 1
EB06562 (1µg/ml) staining of HepG2lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06562 2
HEK293 overexpressing AKT3 (RC224750) and probed with EB06562 (mock transfection in second lane), tested by Origene.
Click here to download PDF datasheet.

Target Protein

Principal Names: RAC-gamma serine/threonine protein kinase, serine threonine protein kinase, Akt-3, protein kinase B gamma, v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma), DKFZP434N0250, RAC-PK-gamma, RAC-gamma, STK-2, PRKBG, PKBG, AKT3
Official Symbol: AKT3
Accession Number(s): NP_005456.1; NP_859029.1
Human GeneID(s): 10000
Important Comments: This antibody is expected to recognise an epitope corresponding to aa 119-133 of both isoforms of human AKT3 protein. This antibody does not cross-react with human AKT1/2.

Immunogen

Peptide with sequence CSPTSQIDNIGEEEM, from the internal region of the protein sequence according to NP_005456.1; NP_859029.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: A 50-55kDa band observed in Human Hepatoblastoma HepG2 lysates (calculated MW of 55.8kDa according to NP_005456 and 54.0kDa according to NP_859029). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Background Reference

Zinda MJ, Johnson MA, Paul JD, Horn C, Konicek BW, Lu ZH, Sandusky G, Thomas JE, Neubauer BL, Lai MT, Graff JR.
AKT-1, -2, and -3 are expressed in both normal and tumor tissues of the lung, breast, prostate, and colon.
Clin Cancer Res. 2001 Aug;7(8):2475-9.
PMID: 11489829

Product Reviews

Please login to review this product.