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EB05049 Goat Anti-ALDH1A1 (C Terminus) Antibody ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog, Cow $300.00 Elite

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EB05049 1
EB05049 staining (0.03µg/ml) of human liver lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
EB05049 2
HEK293 overexpressing ALDH1A1 (RC200723) and probed with EB05049 (mock transfection in first lane), tested by Origene.
Click here to download PDF datasheet.

Target Protein

Principal Names: retinaldehyde dehydrogenase 1, retinal dehydrogenase 1, aldehyde dehydrogenase, liver cytosolic, aldehyde dehydrogenase 1A1, aldehyde dehydrogenase 1, soluble, acetaldehyde dehydrogenase 1, ALDH class 1, RALDH1, PUMB1, MGC2318,, ALDH11, ALDH1, ALDH-E1, ALDC, aldehyde dehydrogenase 1 family, member A1, ALDH1A1
Official Symbol: ALDH1A1
Accession Number(s): NP_000680.2
Human GeneID(s): 216
Non-Human GeneID(s): 11668 (mouse), 24188 (rat)
Important Comments: This antibody may cross-react with ALDH1A2 (GeneID 8854) with one residue difference from this design and with ALDH2 (GeneID 217) with two residues difference from this design.

Immunogen

Peptide with sequence C-EVKTVTVKISQKNS, from the C Terminus of the protein sequence according to NP_000680.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 55kDa band observed in human liver lysates (calculated MW of 54.9kDa according to NP_000680.2). Recommended concentration: 0.03-0.1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Specific References

The goat polyclonal antibody used in this paper was manufactured by us:
Dylla SJ, Beviglia L, Park IK, Chartier C, Raval J, Ngan L, Pickell K, Aguilar J, Lazetic S, Smith-Berdan S, Clarke MF, Hoey T, Lewicki J, Gurney AL.
Colorectal cancer stem cells are enriched in xenogeneic tumors following chemotherapy.
PLoS One. 2008 Jun 18;3(6):e2428.
PMID: 18560594

The goat polyclonal antibody used in this paper was manufactured by us:
Szatmari I, Pap A, Rühl R, Ma JX, Illarionov PA, Besra GS, Rajnavolgyi E, Dezso B, Nagy L.
PPARgamma controls CD1d expression by turning on retinoic acid synthesis in developing human dendritic cells.
J Exp Med. 2006 Oct 2;203(10):2351-62.
PMID: 16982809

The goat polyclonal antibody used in this paper was manufactured by us:
Yoon M, Madden MC, Barton HA.
Developmental expression of aldehyde dehydrogenase in rat: a comparison of liver and lung development.
Toxicol Sci. 2006 Feb;89(2):386-98. Epub 2005 Nov 16.
PMID: 16291827

The goat polyclonal antibody used in this paper was manufactured by us:
Iwata M, Hirakiyama A, Eshima Y, Kagechika H, Kato C, Song SY.
Retinoic acid imprints gut-homing specificity on T cells.
Immunity. 2004 Oct;21(4):527-38.
PMID: 15485630

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