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Goat Anti-ALDH2, Biotinylated Antibody (EB10118-B)

Code Name Applications Availability Size Tested species Price Grade  
EB10118-B Goat Anti-ALDH2, Biotinylated Antibody Pep-ELISA, WB, EIA Yes 100µg specific antibody in 200µl Human, Mouse, Rat $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB10118-B 1
Biotinylated EB10118 (0.3µg/ml) staining of Mouse Liver lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
Click here to download PDF datasheet.

Target Protein

Principal Names: ALDH2, aldehyde dehydrogenase 2 family (mitochondrial), ALDH-E2, ALDHI, ALDM, ALDH class 2, acetaldehyde dehydrogenase 2, liver mitochondrial ALDH, nucleus-encoded mitochondrial aldehyde dehydrogenase 2
Official Symbol: ALDH2
Accession Number(s): NP_000681.2; NP_001191818.1
Human GeneID(s): 217
Non-Human GeneID(s): 11669 (mouse), 29539 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_000681.2; NP_001191818.1).

Immunogen

Peptide with sequence C-DETQFKKILGYIN., from the internal region of the protein sequence according to NP_000681.2; NP_001191818.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 55kDa band observed in Mouse Liver lysates (calculated MW of 56.5kDa according to Mouse NP_033786.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig

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