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Goat Anti-Alpha-synuclein Antibody (EB11713)

Code Name Applications Availability Size Tested species Price Grade  
EB11713 Goat Anti-Alpha-synuclein Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB11713 1
EB11713 (0.1µg/ml) staining of Human Amygdala (A) and fetal Mouse (B) Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB11713 2
EB11713 (5µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: alpha-synuclein, NACP, non A-beta component of AD amyloid, PARK1, PARK4, PD1, SNCA, synuclein alpha-140, synuclein, alpha (non A4 component of amyloid precursor)
Official Symbol: SNCA
Accession Number(s): NP_009292.1; NP_000336.1
Human GeneID(s): 6622
Non-Human GeneID(s): 20617 (mouse), 29219 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_009292.1; NP_000336.1). Reported variants represent identical protein: NP_000336.1, NP_001139527.1, NP_001139526.1

Immunogen

Peptide with sequence C-ATGFVKKDQLGK, from the internal region of the protein sequence according to NP_009292.1; NP_000336.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 17kDa band observed in Human Brain (Amygdala) and fetal Mouse Brain lysates (calculated MW of 14.5kDa according to Human NP_000336.1 and Mouse NP_033247.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig

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