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Goat Anti-Alpha-synuclein (NACP140) Antibody (EB11714)

Code Name Applications Availability Size Tested species Price Grade  
EB11714 Goat Anti-Alpha-synuclein (NACP140) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $315.00 Elite

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EB11714 1
EB11714 (0.1µg/ml) staining of Human Amygdala lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB11714 2
EB11714 (5µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: alpha-synuclein, NACP, non A-beta component of AD amyloid, PARK1, PARK4, PD1, SNCA, synuclein alpha-140, synuclein, alpha (non A4 component of amyloid precursor)
Official Symbol: SNCA
Accession Number(s): NP_000336.1
Human GeneID(s): 6622
Important Comments: This antibody is expected to recognize reported isoform NACP140 (NP_000336.1) only. Reported variants represent identical protein: NP_000336.1, NP_001139527.1, NP_001139526.1

Immunogen

Peptide with sequence C-KDQLGKNEEGAPQE, from the internal region of the protein sequence according to NP_000336.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 17kDa band observed in Human Brain (Amygdala) lysates (calculated MW of 14.5kDa according to NP_000336.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Pig

Specific Reference

This antibody has been successfully used in WB and IF on Rat:
Muñoz P, Cardenas S, Huenchuguala S, Briceño A, Couve E, Paris I, Segura-Aguilar J.
DT-Diaphorase Prevents Aminochrome-Induced Alpha-Synuclein Oligomer Formation and Neurotoxicity.
Toxicol Sci. 2015 May;145(1):37-47.
PMID: 25634539

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