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Goat Anti-Androgen Receptor Antibody (EB06441)

Code Name Applications Availability Size Tested species Price Grade  
EB06441 Goat Anti-Androgen Receptor Antibody Pep-ELISA, WB, IHC, ChIP Yes 100µg specific antibody in 200µl Human $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB06441 1
EB06441(0.3µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06441 2
EB06441 (2µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06441 3
ChIP of 2ug EB06441 with 1ug DHT-treated HEC50 chromatin using the Chromatrap® spin column sonication kit (Protein G) measuring FKBP5 enrichment.
Click here to download PDF datasheet.

Target Protein

Principal Names: androgen receptor, dihydrotestosterone receptor, AR, KD, AIS, TFM, DHTR, SBMA, NR3C4, SMAX1, HUMARA, HYSP1, androgen receptor, dihydrotestosterone receptor, RP11-383C12.1
Official Symbol: AR
Accession Number(s): NP_000035.2
Human GeneID(s): 367
Non-Human GeneID(s): 11835 (mouse), 24208 (rat)
Important Comments: This antibody is expected to recognize isoform 1 (NP_000035.2) only.

Immunogen

Peptide with sequence EVQLGLGRVYPRPPSC, from the N Terminus of the protein sequence according to NP_000035.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 95kDa band observed in Human Brain lysates (calculated MW of 99kDa according to NP_000035.2). Recommended concentration: 0.3-2µg/ml.
IHC: In paraffin embedded Human Prostate shows nuclear staining in the secretory cells of the gland. Recommended concentration: 2-3µg/ml.

Chromatin Immunoprecipitation: A six-fold increased presence of the FKBP5 locus observed when comparing the precipitations using EB06441 with non-specific goat IgG to bring down Androgen Receptor in a chromatin lysate from DHT-stimulated HEC50 cells.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow. This batch is not expected to cross-react with Mouse and Rat in Western blot.

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