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Goat Anti-ANGPT1 Antibody (EB10272)

Code Name Applications Availability Size Tested species Price Grade  
EB10272 Goat Anti-ANGPT1 Antibody Pep-ELISA, WB-Trf Yes 100µg specific antibody in 200µl Human $315.00 Elite

Ordering Information for

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$49.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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  • Purchase order number
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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

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Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB10272 1
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human ANGPT1 with DYKDDDDK tag probed with EB10272(1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/1000) in lane C. Mock-transfected HEK293 probed with EB10272 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: AGP1, AGPT, ANG1, angiopoietin 1, ANGPT1
Official Symbol: ANGPT1
Accession Number(s): NP_001137.2
Human GeneID(s): 284
Non-Human GeneID(s): 11600 (mouse), 89807 (rat)

Immunogen

Peptide with sequence C-PDFSSQKLQH, from the internal region of the protein sequence according to NP_001137.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: In transfected HEK293 transiently expressing full-length Human ANGPT1 (myc and DYKDDDDK tagged), a band of approx. 48kDa was observed. No bands were observed in mock-transfected HEK293 and the 48kDa plus a 75kDa band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 1-2µg/ml.

Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email sales@everestbiotech.com to request datasheet, pricing and delivery information.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig

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