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Code Name Applications Availability Size Tested species Price Grade  
EB11875 Goat Anti-AP2A1 (aa706-727) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB11875 1
EB11875 (0.03µg/ml) staining of Human Frontal cortex lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB11875 2
EB11875 (7µg/ml) staining of paraffin embedded Human Cerebellum. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB11875 3
EB11875 Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: AP2A1, adaptor-related protein complex 2, alpha 1 subunit, ADTAA, AP2-ALPHA, CLAPA1, 100 kDa coated vesicle protein A, AP-2 complex subunit alpha-1, adapter-related protein complex 2 alpha-1 subunit, adaptin, alpha A, adaptor protein complex AP-2 subunit alpha-1, alpha-adaptin A, alpha1-adaptin, clathrin assembly protein complex 2 alpha-A large chain, clathrin-associated/assembly/adaptor protein, large, alpha 1, plasma membrane adaptor HA2/AP2 adaptin alpha A subunit
Official Symbol: AP2A1
Accession Number(s): NP_055018.2
Human GeneID(s): 160
Non-Human GeneID(s): 11771 (mouse), 308578 (rat)
Important Comments: This antibody is expected to recognize reported isoform 1 (NP_055018.2) only.

Immunogen

Peptide with sequence C-ELEPPAPESPMALLADPAPAAD, from the internal region of the protein sequence according to NP_055018.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 110kDa band observed in Human Brain (Frontal cortex) lysates (calculated MW of 108kDa according to NP_055018.2). Recommended concentration: 0.03-0.1µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5-7µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow

Specific References

This antibody (previous batch) has been successfully used in IF on Human:
Dominik P Buser, Gaétan Bader, Martin Spiess
Retrograde transport of CDMPR depends on several machineries as analyzed by sulfatable nanobodies.
Life Sci Alliance. 2022 Jul; 5(7): e202101269.
PMID: 35314489

This antibody (previous batch) has been successfully used in Western blot on Human:
Dominik Pascal Buser, Marie-Françoise Ritz, Suzette Moes; Cristobal Tostado, Stephan Frank, Martin Spiess, Luigi Mariani, Paul Jenö, Jean-Louis Boulay, Gregor Hutter
Quantitative proteomics reveals reduction of endocytic machinery components in gliomas
https://doi.org/10.1016/j.ebiom.2019.07.039
PMID: 31331834

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