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Goat Anti-APOBEC3C (C-term) Antibody (EB08307)

Related Products: EBP08307 - Immunizing Peptide, EBP08333 - Immunizing Peptide, Goat Anti-APOBEC3C Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB08307 Goat Anti-APOBEC3C (C-term) Antibody Pep-ELISA, WB, FC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc
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EB08307 1
EB08307 (0.5µg/ml) staining of Daudi cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB08307 2
EB08307 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: APOBEC3C, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3C, APOBEC1L, ARDC2, ARDC4, ARP5, MGC19485, PBI, bK150C2.3
Official Symbol: APOBEC3C
Accession Number(s): NP_055323.2
Human GeneID(s): 27350

Immunogen

Peptide with sequence C-RLLKRRLRESLQ, from the C terminus of the protein sequence according to NP_055323.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx. 22kDa band observed in lysates of cell line Daudi (calculated MW of 22.8kDa according to NP_055323.2). Recommended concentration: 0.5-1.5µg/ml. Primary incubation 1 hour at room temperature. This antibody has been successfully used in WB on Human: https://doi.org/10.1101/2021.01.13.426487.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Specific References

This antibody has been successfully used in the following paper:
Camille Libre, Tanja Seissler, Santiago Guerrero, Julien Batisse, Cédric Verriez, Benjamin Stupfler, Orian Gilmer, Romina Cabrera-Rodriguez, Melanie M Weber, Agustin Valenzuela-Fernandez, Andrea Cimarelli, Lucie Etienne, Roland Marquet, Jean-Christophe Paillart
A conserved uORF regulates APOBEC3G translation and is targeted by HIV-1 Vif protein to repress the antiviral factor
Biomedicines. 2021 Dec 22;10(1):13.
PMID: 35052693

This antibody has been successfully used in Western blot on Human:
Camille Libre, Tanja Seissler, Santiago Guerrero, Julien Batisse, Cédric Verriez, Benjamin Stupfler, Orian Gilmer, Melanie M. Weber, Andrea Cimarelli, Lucie Etienne, Roland Marquet & Jean-Christophe Paillar
A conserved uORF impacts APOBEC3G translation and is essential for translational inhibition by the HIV-1 Vif protein
https://doi.org/10.1101/2021.01.13.426487; this version posted January 13, 2021.
PMID: 0

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