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Goat Anti-ARFGAP3 Antibody (EB05228)

Related Products: EBP05228 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB05228 Goat Anti-ARFGAP3 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog, Cow $315.00 Elite

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EB05228 1
EB05228 staining (2µg/ml) of mouse brain extracts (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: FLJ45618, OTTHUMP00000028526, ADP-ribosylation factor GTPase activating protein 1, RP1-47A17.3, ARFGAP1, ADP-ribosylation factor GTPase activating protein 3, ARFGAP3
Official Symbol: ARFGAP3
Accession Number(s): NP_055385.3; NP_001135765.1
Human GeneID(s): 26286
Important Comments: This antibody is expected to recognize both reported isoforms (NP_055385.3; NP_001135765.1).

Immunogen

Peptide with sequence C-NGVVTSIQDRYGS, from the C Terminus of the protein sequence according to NP_055385.3; NP_001135765.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 55-60kDa band seen in mouse brain extracts. Recommended for use at 1-3µg/ml.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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