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Goat Anti-Argininosuccinate synthetase 1, Biotinylated Antibody (EB07697-B)

Code Name Applications Availability Size Tested species Price Grade  
EB07697-B Goat Anti-Argininosuccinate synthetase 1, Biotinylated Antibody Pep-ELISA, WB, IF, IP, IHC Yes 100µg specific antibody in 200µl Human, Cow $315.00 Elite

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB07697-B 1
Biotinylated EB07697 (0.03µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
Click here to download PDF datasheet.

Target Protein

Principal Names: ASS1, argininosuccinate synthase 1, ASS, CTLN1, argininosuccinate synthetase 1, citrulline--aspartate ligase, citrulline-aspartate ligase
Official Symbol: ASS1
Accession Number(s): NP_000041.2
Human GeneID(s): 445
Non-Human GeneID(s): 11898 (mouse), 25698 (rat)
Important Comments: Reported variants represent identical protein: NP_446464.1, NP_000041.2

Immunogen

Peptide with sequence C-ENPKNQAPPGLYTKTQD., from the internal region of the protein sequence according to NP_000041.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 46kDa band observed in Human Kidney lysates (calculated MW of 46.5kDa according to NP_000041.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.01-0.03µg/ml.

Species Reactivity

Tested: Human, Cow
Expected from sequence similarity: Human, Mouse, Rat, Dog

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