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Goat Anti-ARHU / WRCH1 Antibody (EB05375)

Code Name Applications Availability Size Species reactivity Price Grade  
EB05375 Goat Anti-ARHU / WRCH1 Antibody Pep-ELISA Yes 100µg specific antibody in 200µl Human $210.00 Aspiring

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Orders are placed with and invoiced by our US partner Abcore LLC.

 

Target Protein

Principal Names: ras-like gene family member U, GTP-binding protein like 1, GTP-binding protein SB128, WRCH1, FLJ10616, Wnt-1 responsive Cdc42 homolog, GTP-binding protein like 1, CDC42-like GTPase, Ryu GTPase, ras homolog gene family, member U, 2310026M05Rik, fJ646B12.2, DJ646B12.2, FLJ10616, CDC42L1, WRCH-1, hG28K, WRCH1, ARHU, ras homolog gene family, member U, RHOU
Official Symbol: RHOU
Accession Number(s): NP_067028.1
Human GeneID(s): 58480

Immunogen

Peptide with sequence PPQQGDPAFPDRCEA, from the N Terminus of the protein sequence according to NP_067028.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Preliminary experiments gave an approx. 22kDa band in lysates of cell lines A431, HeLa and HEK293 after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 28.2kDa according to NP_067028.1. The 22kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested:
Expected from sequence similarity: Human

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