Change country
Tel: 888-320-GOAT (4628)
Fax: 888-841-9041
Home

Goat Anti-ARIH1 / HHARI Antibody (EB05812)

Code Name Applications Availability Size Species reactivity Price Grade  
EB05812 Goat Anti-ARIH1 / HHARI Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Cow $315.00 Elite

Ordering Information for

Order direct from Everest

$40.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA.

Order offline

Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are placed with and invoiced by our US partner Abcore LLC.

 

EB05812 1
EB05812 staining (0.5µg/ml) of U937 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ariadne ubiquitin-conjugating enzyme E2 binding protein homolog 1, FLJ93118, FLJ20329, DKFZp686O13120, ariadne, Drosophila, homolog of, ariadne homolog, ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila), UBCH7BP, HARI, ARI, HHARI, ARIH1
Official Symbol: ARIH1
Accession Number(s): NP_005735.2
Human GeneID(s): 25820
Non-Human GeneID(s): 23806 (mouse)

Immunogen

Peptide with sequence C-HEGYEKDLWEYIED, from the C Terminus of the protein sequence according to NP_005735.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 65kDa band seen in U937 lysate [Predicted MW of approx. 61kDa according to NP_005735]. Recommended for use at 0.2-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Cow

Specific References

The goat polyclonal antibody used in this paper was manufactured by us:
LaVoie MJ, Cortese GP, Ostaszewski BL, Schlossmacher MG.
The effects of oxidative stress on parkin and other E3 ligases.
J Neurochem. 2007 Dec;103(6):2354-68.
PMID: 17883392

The goat polyclonal antibody used in this paper was manufactured by us:
Wong ES, Tan JM, Wang C, Zhang Z, Tay SP, Zaiden N, Ko HS, Dawson VL, Dawson TM, Lim KL.
Relative sensitivity of parkin and other cysteine-containing enzymes to stress-induced solubility alterations.
J Biol Chem. 2007 Apr 20;282(16):12310-8.
PMID: 17329252

Product Reviews

Please login to review this product.