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Goat Anti-ARIH2 / TRIAD1 Antibody (EB05813)

Code Name Applications Availability Size Species reactivity Price Grade  
EB05813 Goat Anti-ARIH2 / TRIAD1 Antibody ELISA, WB, IF, IP Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog, Cow $300.00 Elite

Ordering Information for

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Now shipping direct to US customers! All orders will be shipped to you by our logistics partner in Kentucky. Almost all products are held in stock for immediate shipment, see individual product pages for availability. Orders are placed with and invoiced by Everest Biotech Ltd in the United Kingdom.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (sales@everestbiotech.com), fax (888-841-9041), phone (888-320-GOAT) or mail. Please include the following information with your order:

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Order from our local distributor

Axxora, LLC

6181 Cornerstone Court East Suite 103,
San Diego
CA 92121-4727
USA

Tel: 858-550-8830
Toll Free Tel: 1-800-550-3033
Fax: 858-550-8825
Toll Free Fax: 1-800-550-8825
Email: axxora-usa@axxora.com
Website: www.axxora.com

Target Protein

Principal Names: ariadne homolog 2, OTTHUMP00000210388, FLJ33921, FLJ10938, ariadne-2 (D. melanogaster) homolog (all-trans retinoic acid inducible RING finger), all-trans retinoic acid inducible RING finger, TRIAD1, ARI2, ariadne homolog 2 (Drosophila), ARIH2
Official Symbol: ARIH2
Accession Number(s): NP_006312.1
Human GeneID(s): 10425
Non-Human GeneID(s): 23807 (mouse)

Immunogen

Peptide with sequence C-EQRRRTLLKDFHDT, from the C Terminus of the protein sequence according to NP_006312.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Customer's experiments gave a band at approx 60kDa in lysates in both Human HeLa and Mouse Muscle (IM2 & C2C12) cells. This band disappeared after RNAi knock-down. Calculated MW of 57.8kDa according to NP_006312.1). Recommended concentration: 1-3µg/ml.

Customer's experiments showed nuclear staining in DAPI with Immunofluorescence. AB works well in Immunoprecipitation.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Background Reference

van der Reijden BA, Erpelinck-Verschueren CA, Lowenberg B, Jansen JH.
TRIADs: a new class of proteins with a novel cysteine-rich signature.
Protein Sci. 1999 Jul;8(7):1557-61.
PMID: 10422847

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