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Goat Anti-ARL4A Antibody (EB05549)

Related Products: EBP05549 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB05549 Goat Anti-ARL4A Antibody ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Pig $300.00 Elite

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Now shipping direct to US customers! All orders will be shipped to you by our logistics partner in Kentucky. Almost all products are held in stock for immediate shipment, see individual product pages for availability. Orders are placed with and invoiced by Everest Biotech Ltd in the United Kingdom.

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CA 92121-4727
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Toll Free Tel: 1-800-550-3033
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EB05549 1
EB05549 staining (2µg/ml) of Human Testis lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: OTTHUMP00000201575, OTTHUMP00000155200, ADP-ribosylation factor-like 4A, ADP-ribosylation factor-like 4, ARL4, ARL4A
Official Symbol: ARL4A
Accession Number(s): NP_001032241.1; NP_005729.1; P_997625.1
Human GeneID(s): 10124
Non-Human GeneID(s): 11861 (mouse)
Important Comments: This is also expected to react with a hypothetical human protein, XP_166703, and a hypothetical mouse protein, AAH03725 - both very similar to ARL4.

Immunogen

Peptide with sequence GNGLSDQTSILSN-C, from the N Terminus of the protein sequence according to NP_001032241.1; NP_005729.1; P_997625.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 23kDa band seen in Human Testis lysate. Recommended for use at 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Pig

Background Reference

Jacobs S, Schurmann A, Becker W, Bockers TM, Copeland NG, Jenkins NA, Joost HG.
The mouse ADP-ribosylation factor-like 4 gene: two separate promoters direct specific transcription in tissues and testicular germ cell.
Biochem J 1998 Oct 15;335 ( Pt 2):259-65
PMID: 9761722

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