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Goat Anti-ARPC3 Antibody (EB06354)

Related Products: EBP06354 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB06354 Goat Anti-ARPC3 Antibody ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog, Cow $300.00 Elite

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EB06354 1
EB06354 staining (1µg/ml) of Mouse Brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: actin related protein 2/3 complex subunit 3, ARP2/3 protein complex subunit p21, actin related protein 2/3 complex, subunit 3, 21kDa, p21-Arc, ARC21, ARPC3
Official Symbol: ARPC3
Accession Number(s): NP_005710.1
Human GeneID(s): 10094
Non-Human GeneID(s): 56378 (mouse)
Important Comments: Please note there are two hypothetical proteins called "similar to the Arp2/3 protein complex subunit p21-Arc" that are virtually identical (CAC14083, XP_208062).

Immunogen

Peptide with sequence C-RQFMNKSLSGPGQ, from the C Terminus of the protein sequence according to NP_005710.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Band observed just below 20kDa marker in Hela, Human Brain and Mouse Brain lysates (predicted MW of 20kDa according to NP_005710). Recommended for use at 1-3µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Background Reference

Welch MD, DePace AH, Verma S, Iwamatsu A, Mitchison TJ.
The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly.
J Cell Biol. 1997 Jul 28;138(2):375-84.
PMID: 9230079

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