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Goat Anti-Arrestin beta 2 Antibody (EB06745)

Related Products: EBP06745 - Immunizing Peptide, Goat Anti-Arrestin beta 2 Antibody - Trial
Code Name Applications Availability Size Species reactivity Price Grade  
EB06745 Goat Anti-Arrestin beta 2 Antibody ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $300.00 Elite

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EB06745 1
EB06745 (0.3µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: BARR2, beta-arrestin 2, arrestin beta 2, DKFZp686L0365, ARR2, ARB2, HGNC:712, ARRB2, arrestin, beta 2
Official Symbol: ARRB2
Accession Number(s): NP_004304.1; NP_945355.1
Human GeneID(s): 409
Important Comments: This antibody is expected to recognise both reported isoforms NP_004304.1 and NP_945355.1. No crossreactivity is expected with Arrestin beta 1.

Immunogen

Peptide with sequence C-HDHIPLPRPQS, from the internal region of the protein sequence according to NP_004304.1; NP_945355.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 40kDa band observed in Human Brain lysates (calculated MW of 46.1kDa according to NP_004304.1 and 44.4 according to NP_945355.1). Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

Background Reference

Lefkowitz RJ, Shenoy SK.
Transduction of receptor signals by beta-arrestins.
Science. 2005 Apr 22;308(5721):512-7. Review.
PMID: 15845844

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