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Code Name Applications Availability Size Tested species Price Grade  
EB06745 Goat Anti-Arrestin beta 2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB06745 1
EB06745 (0.3µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06745 2
EB06745 (4µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: arrestin, beta 2, ARRB2, HGNC:712, ARB2, ARR2, DKFZp686L0365, arrestin beta 2, beta-arrestin 2, BARR2, arrestin 3, arrestin beta-2
Official Symbol: ARRB2
Accession Number(s): NP_004304.1; NP_945355.1; NP_001244257.1; NP_001244259.1; NP_001244260.1
Human GeneID(s): 409
Non-Human GeneID(s): 216869 (mouse), 25388 (rat)
Important Comments: This antibody is expected to recognise both reported isoforms 1, 2, 3, 5 and 6 (NP_004304.1; NP_945355.1; NP_001244257.1; NP_001244259.1; NP_001244260.1). No crossreactivity is expected with Arrestin beta 1.

Immunogen

Peptide with sequence C-HDHIPLPRPQS, from the internal region of the protein sequence according to NP_004304.1; NP_945355.1; NP_001244257.1; NP_001244259.1; NP_001244260.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 40kDa band observed in Human Brain lysates (calculated MW of 46.1kDa according to NP_004304.1 and 44.4 according to NP_945355.1). Recommended concentration: 0.3-1µg/ml. A customer found positive results in WB on Rat aortic smooth muscle. A customer found positive results in WB on Mouse smooth muscle. A customer found positive results in WB on Mouse aortic smooth muscle. A customer found positive results in WB on Human colon adenocarcinoma cells (SW480).
IHC: In paraffin embedded Human Cerebellum shows mainly nuclear staining of purkinje cells and neuronal cells in the Molecular Layer. Recommended concentration: 4-6µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat

Specific References

This antibody has been successfully used inWestern blot in Mouse:
Napolitano F, Pasqualetti M, Usiello A, Santini E, Pacini G, Sciamanna G, Errico F, Tassone A, Di Dato V, Martella G, Cuomo D, Fisone G, Bernardi G, Mandolesi G, Mercuri NB, Standaert DG, Pisani A.
Dopamine D2 receptor dysfunction is rescued by adenosine A2A receptor antagonism in a model of DYT1 dystonia.
Neurobiol Dis. 2010 Jun;38(3):434-45.
PMID: 20227500

This antibody has been successfully used in IP in Rat:
Zakrzewicz A, Krasteva G, Wilhelm J, Dietrich H, Wilker S, Padberg W, Wygrecka M, Grau V.
Reduced expression of arrestin beta 2 by graft monocytes during acute rejection of rat kidneys.
Immunobiology. 2011 Jul;216(7):854-61.
PMID: 21193245

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