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Goat Anti-Arylsulfatase D Antibody (EB08469)

Related Products: EBP08469 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08469 Goat Anti-Arylsulfatase D Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB08469 1
EB08469 (0.3µg/ml) staining of A459 lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: arylsulfatase D, ARSD
Official Symbol: ARSD
Accession Number(s): NP_001660.2; NP_033667.2
Human GeneID(s): 414
Important Comments: This antibody is expected to recognise both reported isoforms (NP_001660.2; NP_033667.2)

Immunogen

Peptide with sequence C-DASNGYRALQWNA, from the internal region of the protein sequence according to NP_001660.2; NP_033667.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 60+40kDa bands observed in lysates of cell lines A549 and HepG2 (calculated MW of 64.9kDa according to NP_001660.2 and of 42.3 according to NP_033667.2). Recommended concentration: 0.3-1µg/ml. An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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