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Goat Anti-ATF2 Antibody (EB05198)

Code Name Applications Availability Size Tested species Price Grade  
EB05198 Goat Anti-ATF2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $315.00 Elite

Ordering Information for

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB05198 1
EB05198 staining (0.5µg/ml) of Human Placenta lysate (RIPA buffer, 35µg total protein per lane). Detected by chemiluminescence.
EB05198 2
EB05198 (1µg/ml) staining of HepG2 (A), K562 (B) and HeLa (C) nuclear lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ATF2, activating transcription factor 2, HB16, CREB2, TREB7, CRE-BP1, cAMP responsive element binding protein 2, MGC111558, activating transcription factor 2 splice variant ATF2-var2, cAMP responsive element binding protein 2, formerly
Official Symbol: ATF2
Accession Number(s): NP_001871.2
Human GeneID(s): 1386
Non-Human GeneID(s): 81647 (rat)

Immunogen

Peptide with sequence C-IVMAPSSQSQPSGS, from the C Terminus of the protein sequence according to NP_001871.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 55kDa and 30kDa bands observed in lysates of Human Placenta. These bands correspond to earlier findings in literature (Bailey et al , J Clin Endocrinol Metab. 2002 Apr;87(4):1717-28.; PMID: 11932306). Approx 75kDa observed in nuclear lysates of cell lines HeLa, HepG2 and K562 (calculated MW of 54.5kDa according to NP_001871.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Knippen et al, Anticancer Res. 2009 Jan;29(1):183-189 ; PMID: 19331149). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Rat, Dog, Cow

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