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Goat Anti-ATF2 Antibody (EB05198)

Related Products: EBP05198 - Immunizing Peptide, Goat Anti-ATF2 Antibody - Trial
Code Name Applications Availability Size Species reactivity Price Grade  
EB05198 Goat Anti-ATF2 Antibody ELISA, WB Yes 100µg specific antibody in 200µl Human, Rat, Dog $300.00 Elite

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EB05198 1
EB05198 staining (0.5µg/ml) of Human Placenta lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: cAMP responsive element binding protein 2, formerly, activating transcription factor 2 splice variant ATF2-var2, MGC111558, cAMP responsive element binding protein 2, CRE-BP1, TREB7, CREB2, HB16, activating transcription factor 2, ATF2
Official Symbol: ATF2
Accession Number(s): NP_001871.2
Human GeneID(s): 1386
Non-Human GeneID(s): 81647 (rat)

Immunogen

Peptide with sequence C-IVMAPSSQSQPSGS, from the C Terminus of the protein sequence according to NP_001871.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Experiments gave bands at approx 55kDa and 30kDa in lysates of Human Placenta and of cell line HeLa after 0.5µg/ml antibody staining. These bands correspond to earlier findings in literature with different antibodies (Bailey et al , J Clin Endocrinol Metab. 2002 Apr;87(4):1717-28.; PMID: 11932306). This protein has a calculated MW of 54.5kDa according to NP_001871.2. Recommended concentration: 0.5-1.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Rat, Dog

Background Reference

Monzen K, Hiroi Y, Kudoh S, Akazawa H, Oka T, Takimoto E, Hayashi D, Hosoda T, Kawabata M, Miyazono K, Ishii S, Yazaki Y, Nagai R, Komuro I.
Smads, TAK1, and their common target ATF-2 play a critical role in cardiomyocyte differentiation.
J Cell Biol 2001 May 14;153(4):687-98
PMID: 11352931

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