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Goat Anti-ATF4 Antibody (EB05247)

Code Name Applications Availability Size Species reactivity Price Grade  
EB05247 Goat Anti-ATF4 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Pig. This batch is not expected to cross-react with Mouse and Pig in Western blot. $315.00 Elite

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Labscoop

Little Rock, Arkansas
P.O. Box 242164

Email: support@labscoop.com
Website: https://www.labscoop.com/
Phone: 800.316.3081
Fax: 800.316.3081

EB05247 1
EB05247 (0.3µg/ml) staining of lysate of cell line K562 (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ATF4, activating transcription factor 4 (tax-responsive enhancer element B67), CREB2, TXREB, CREB-2, TAXREB67, OTTHUMP00000199130, activating transcription factor 4, cAMP response element-binding protein 2
Official Symbol: ATF4
Accession Number(s): NP_001666.2; NP_877962.1
Human GeneID(s): 468
Non-Human GeneID(s): 11911 (mouse), 79255 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001666.2; NP_877962.1).

Immunogen

Peptide with sequence C-EEVRKARGKKRVP, from the C Terminus of the protein sequence according to NP_001666.2; NP_877962.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 37kDa band observed in lysate of K562 cell lines (calculated MW of 38.6kDa according to NP_001666.2 and of NP_877962.1). Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Pig. This batch is not expected to cross-react with Mouse and Pig in Western blot.

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