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Goat Anti-B5 Receptor / PCID1 / EIF3M Antibody (EB06955)

Related Products: EBP06955 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB06955 Goat Anti-B5 Receptor / PCID1 / EIF3M Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog, Cow $315.00 Elite

Ordering Information for

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be processed and invoiced by our US partner Abcore LLC.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are placed with and invoiced by our US partner Abcore LLC.

 
EB06955 1
EB06955 (0.1µg/ml) staining of A549 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06955 2
EB06955 (5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PCID1, B5, eukaryotic translation initiation factor 3, subunit M, EIF3M, fetal lung protein B5, dendritic cell protein, hfl-B5, GA17, FLJ29030, PCI domain containing 1 (herpesvirus entry mediator), PCID1, B5 receptor
Official Symbol: EIF3M
Accession Number(s): NP_006351.2
Human GeneID(s): 10480
Non-Human GeneID(s): 98221 (mouse), 295975 (rat)

Immunogen

Peptide with sequence C-NAWKQNLNKVKN, from the C Terminus of the protein sequence according to NP_006351.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 38-40kDa band observed in lysates of human lung carcinoma cell line A549 (calculated MW of 42.5kDa according to NP_006351.2). Recommended concentration: 1-3µg/ml.
IHC: In paraffin embedded Human Testis shows strong staining of cytoplasm in select spermatocytes. Recommended concentration, 5-10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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