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Code Name Applications Availability Size Tested species Price Grade  
EB05487 Goat Anti-BAG5 (N Terminus) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com

EB05487 1
EB05487 (2µg/ml) staining of nuclear HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB05487 2
EB05487 (3.75µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: BAG5, BCL2-associated athanogene 5, BAG-5, KIAA0873, BAG-family molecular chaperone regulator-5
Official Symbol: BAG5
Accession Number(s): NP_001015049.1; NP_004864.1; NP_001015048.1
Human GeneID(s): 9529
Important Comments: Variants (NP_004864.1; NP_001015048.1) encode the same isoform.

Immunogen

Peptide with sequence DMGNQHPSISRLQ-C, from the N Terminus of the protein sequence according to NP_001015049.1; NP_004864.1; NP_001015048.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 55+48kDa bands observed in nuclear lysates of cell line HeLa (calculated MW of 56.0kDa according to NP_001015049.1 and of 51.2kDa according to NP_004864.1; NP_001015048.1). Recommended concentration: 1-3µg/ml.
IHC: Paraffin embedded Human Testis. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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