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Goat Anti-BDNF Antibody (EB08117)

Code Name Applications Availability Size Tested species Price Grade  
EB08117 Goat Anti-BDNF Antibody Pep-ELISA, IHC, IF Yes 100µg specific antibody in 200µl Human, Mouse, Rat $315.00 Elite

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EB08117 1
EB08117 (1µg/ml) staining of PFA-perfused cryosection of Mouse sciatic nerve (left) and injured sciatic nerve (right). Alexa 488-staining. Data obtained from Simon Glerup, Department of Biomedicine Aarhus University, Denmark.
EB08117 2
EB08117 (5ug/ml) staining of paraffin embedded Rat Cerebral Cortex (A) and Rat Hippocampus (B), CRY-staining with DAPI nuclear counter stain in blue. Antigen retrieval with citrate buffer pH 6 at 95-98C.
EB08117 3
EB08117 (5ug/ml) staining of paraffin embedded Rat Cerebral Cortex. Antigen retrieval with citrate buffer pH 6 at 95-98C, DAB-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: BDNF, brain-derived neurotrophic factor, MGC34632, neurotrophin
Official Symbol: BDNF
Accession Number(s): NP_001700.2; NP_001700.2; NP_733928.1; NP_733929.1; NP_733930.1; NP_733931.1
Human GeneID(s): 627
Important Comments: This antibody is expected to recognise all reported isoforms (NP_001700.2; NP_001700.2; NP_733928.1; NP_733929.1; NP_733930.1; NP_733931.1)

Immunogen

Peptide with sequence ETKCNPMGYTKE, from the internal region of the protein sequence according to NP_001700.2; NP_001700.2; NP_733928.1; NP_733929.1; NP_733930.1; NP_733931.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
IHC: In paraffin embedded Human Cortex shows cytoplasm staining of neuronal cells. Recommended concentration, 3-5µg/ml. Frozen sections of Mouse sciatic nerve shows intensified staining after injury, consistent with the literature (Tonra et al, J Neurosci. 1998 Jun 1;18(11):4374-83. PMID: 9592114). Recommended concentration, 1-3µg/ml. Based on the mouse data, this antibody is endorsed by pAbmAbs (http://pabmabs.com/wordpress/?p=1596). In paraffin embedded Rat Cerebral Cortex and Rat Hippocampus show cytoplasm staining in neurons. Recommended concentration 3-5ug/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog

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