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Goat Anti-BDNF Antibody (EB08117)

Code Name Applications Availability Size Tested species Price Grade  
EB08117 Goat Anti-BDNF Antibody Pep-ELISA, IF Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com

EB08117 1
EB08117 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing speckled nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB08117 2
EB08117 (1µg/ml) staining of PFA-perfused cryosection of Mouse sciatic nerve (left) and injured sciatic nerve (right). Alexa 488-staining. Data obtained from Simon Glerup, Department of Biomedicine, Aarhus University, Denmark.This data is from a previous batch, not on sale.
EB08117 3
EB08117 (5ug/ml) staining of paraffin embedded Rat Cerebral Cortex (A) and Rat Hippocampus (B), CRY-staining with DAPI nuclear counter stain in blue. Antigen retrieval with citrate buffer pH 6 at 95-98C. This data is from a previous batch, not on sale.
Click here to download PDF datasheet.

Target Protein

Principal Names: BDNF, brain-derived neurotrophic factor, MGC34632, neurotrophin
Official Symbol: BDNF
Accession Number(s): NP_001700.2; NP_001700.2; NP_733928.1; NP_733929.1; NP_733930.1; NP_733931.1
Human GeneID(s): 627
Important Comments: This antibody is expected to recognise all reported isoforms (NP_001700.2; NP_001700.2; NP_733928.1; NP_733929.1; NP_733930.1; NP_733931.1)

Immunogen

Peptide with sequence ETKCNPMGYTKE, from the internal region of the protein sequence according to NP_001700.2; NP_001700.2; NP_733928.1; NP_733929.1; NP_733930.1; NP_733931.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.

Immunofluorescence: Expression of the protein seen in the nucleus and cytoplasm of Glioblastoma U251 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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