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Code Name Applications Availability Size Species reactivity Price Grade  
EB05676 Goat Anti-BOB1 / OCAB / OBF1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog, Cow $315.00 Elite

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EB05676 1
EB05676 staining (2µg/ml) of mouse spleen lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: OCAB, POU class 2 associating factor 1, POU domain, class 2, associating factor 1, OBF-1, POU2AF1, OBF1, BOB1
Official Symbol: POU2AF1
Accession Number(s): NP_006226.2
Human GeneID(s): 5450
Non-Human GeneID(s): 18985 (mouse)

Immunogen

Peptide with sequence C-DAYALNHTLSVEGF, from the C Terminus of the protein sequence according to NP_006226.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 30kDa band seen in mouse spleen lysate (Predicted MW of 28kDa according to NP_006226). Recommended for use at 1-3µg/ml.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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