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Goat Anti-BRF2 Antibody (EB05668)

Code Name Applications Availability Size Species reactivity Price Grade  
EB05668 Goat Anti-BRF2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $315.00 Elite

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EB05668 1
EB05668 staining (1.5µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: RNA polymerase III transcription initiation factor BRF2, RNA polymerase III transcription initiation factor BRFU, transcription factor IIB- related factor, TFIIIB50, TFIIIB50, FLJ11052, BRFU, BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like, BRF2
Official Symbol: BRF2
Accession Number(s): NP_060780.2
Human GeneID(s): 55290

Immunogen

Peptide with sequence C-QAARQAATSVPNPP, from the C Terminus of the protein sequence according to NP_060780.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 50kDa band observed in Hela and Human Placenta lysates (predicted MW of 48kDa according to NP_060780). Recommended for use at 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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