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Goat Anti-Calnexin Antibody (EB09525)

Code Name Applications Availability Size Tested species Price Grade  
EB09525 Goat Anti-Calnexin Antibody Pep-ELISA, WB, IHC, EIA Yes 100µg specific antibody in 200µl Human, Mouse $315.00 Elite

Ordering Information for

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB09525 1
EB09525 (0.3µg/ml) staining of Mouse Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB09525 2
EB09525 (3.8µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB09525 3
EB09525 (3.75µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB09525 4
EB09525 (3.75µg/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB09525 5
EB09525 (1.5ug/ml) as the reporter with EB002024 as the capture rabbit antibody (2.5ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: CANX, calnexin, CNX, FLJ26570, IP90, P90, major histocompatibility complex class I antigen-binding protein p88
Official Symbol: CANX
Accession Number(s): NP_001737.1
Human GeneID(s): 821
Non-Human GeneID(s): 12330 (mouse), 29144 (rat)
Important Comments: Reported variants represent identical protein (NP_001019820.1, NP_001737.1).

Immunogen

Peptide with sequence C-SKTPELNLDQFHDKT, from the internal region (near N Terminus) of the protein sequence according to NP_001737.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 100kDa band observed in Mouse Heart, Human Brain (Cerebellum), NIH3T3 and HeLa lysates (calculated MW of 67.6kDa according to Human NP_001737.1 and 67.2kDa according to Mouse NP_001103969.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Oliver et al., J Biol Chem. 1996 Jun 7;271(23):13691-6., PMID: 8662691). Recommended concentration: 0.3-1µg/ml.
IHC: In paraffin embedded Human Kidney shows textured staining in the cytoplasm of DCT cells. Recommended concentration: 3-6µg/ml. Paraffin embedded Human Testis and Uterus. Recommended concentration: 3.75µg/ml.

Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant Calnexin captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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