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Goat Anti-Catalase / CAT Antibody (EB07282)

Code Name Applications Availability Size Tested species Price Grade  
EB07282 Goat Anti-Catalase / CAT Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB07282 1
EB07282 (0.1µg/ml) staining of Human Kidney (A) and Liver (B) and Mouse Kidney (C) and Liver (D) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB07282 2
EB07282 (2µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
EB07282 3
EB07282 (2µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
Click here to download PDF datasheet.

Target Protein

Principal Names: CAT, catalase, HGNC:1516, MGC138422, MGC138424
Official Symbol: CAT
Accession Number(s): NP_001743.1
Human GeneID(s): 847
Non-Human GeneID(s): 12359 (mouse)

Immunogen

Peptide with sequence C-DKYNAEKPKNAIHT, from the internal region of the protein sequence according to NP_001743.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 60kDa band observed in Human Kidney lysates (calculated MW of 59.8kDa according to NP_001743.1). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Dog

Specific Reference

This antibody has been successfully used in Western blot on Mouse:
Patche J, Girard D, Catan A, Boyer F, Dobi A, Planesse C, Diotel N, Guerin-Dubourg A, Baret P, Bravo SB, Paradela-Dobarro B, Álvarez E, Essop MF, Meilhac O, Bourdon E, Rondeau P.
Diabetes-induced hepatic oxidative stress: a new pathogenic role for glycated albumin.
Free Radic Biol Med. 2017 Jan;102:133-148.
PMID: 27890722

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