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Goat Anti-Catalase / CAT Antibody (EB07282)

Related Products: EBP07282 - Immunizing Peptide, Goat Anti-Catalase / CAT Antibody - Trial
Code Name Applications Availability Size Species reactivity Price Grade  
EB07282 Goat Anti-Catalase / CAT Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Dog $315.00 Elite

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EB07282 1
EB07282 (2µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB07282 2
EB07282 (1µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: MGC138424, MGC138422, HGNC:1516, catalase, CAT
Official Symbol: CAT
Accession Number(s): NP_001743.1
Human GeneID(s): 847
Non-Human GeneID(s): 12359 (mouse)

Immunogen

Peptide with sequence C-DKYNAEKPKNAIHT, from the internal region of the protein sequence according to NP_001743.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 60kDa band observed in Human, Mouse and Rat Kidney and Liver lysates (calculated MW of 59.8kDa according to NP_001743.1). Recommended concentration: 0.3-1µg/ml.
IHC: Immunohistochemistry: In paraffin embedded Human Liver shows vesicular staining in the cytoplasm of hepatocytes. Recommended concentration, 2-4µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Dog

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