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Goat Anti-CCAR2 / DBC1 (aa842-855) Antibody (EB12588)

Related Products: EBP12588 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB12588 Goat Anti-CCAR2 / DBC1 (aa842-855) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping (not applicable to SB-shifix) to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

  • Product code(s)
  • Purchase order number
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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB12588 1
EB12588 (0.5µg/ml) staining of HEK293 (A) and MCF7 (B) nuclear lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB12588 2
EB12588 (2µg/ml) staining of NIH3T3 nuclear lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: CCAR2, cell cycle and apoptosis regulator 2, DBC-1, DBC1, KIAA1967, NET35, p30 DBC, p30DBC, cell cycle and apoptosis regulator protein 2, cell division cycle and apoptosis regulator protein 2, deleted in breast cancer 1, p30 DBC protein
Official Symbol: CCAR2
Accession Number(s): NP_066997.3
Human GeneID(s): 57805
Non-Human GeneID(s): 219158 (mouse), 306007 (rat)

Immunogen

Peptide with sequence C-ESHNRFSATEVTNK, from the internal region of the protein sequence according to NP_066997.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 140kDa band observed in nuclear lysates of cell lines HEK293, MCF7 and NIH3T3 (calculated MW of 103kDa according to Human NP_066997.3 and Mouse NP_666167.3). The observed molecular weight is explained by its acetylation (Zheng et al, Mol Cell Biol. 2013 Dec;33(24):4960-70. PMID: 24126058). Recommended concentration: 0.5-2µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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