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Goat Anti-CCKAR Antibody (EB06768)

Code Name Applications Availability Size Tested species Price Grade  
EB06768 Goat Anti-CCKAR Antibody Pep-ELISA, IHC, ICC, IF Yes 100µg specific antibody in 200µl Human, Rat $315.00 Elite

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EB06768 1
EB06768 (10µg/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CCKAR, cholecystokinin A receptor, HGNC:1570, CCK-A, CCK1-R, CCKRA, cholecystokinin-1 receptor, cholecystokinin type-A receptor
Official Symbol: CCKAR
Accession Number(s): NP_000721.1
Human GeneID(s): 886
Non-Human GeneID(s): 12425 (mouse), 24889 (rat)


Peptide with sequence C-FEASQKKSAKERKPST, from the internal region of the protein sequence according to NP_000721.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Preliminary experiments in Human Pancreas lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml).
IHC: In paraffin embedded Human Pancreas, shows cytoplasmic staining of some cells in the islets of Langerhans. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human

Specific Reference

This antibody has been successfully used in ICC/IF on RIN-14B cells:
El-Kouhen K, Morisset J.
Control of somatostatin (SS) secretion by CCK-1 and CCK-2 receptors' occupation in RIN-14B cells, a rat pancreatic islet cell line.
Pancreas. 2010 Mar;39(2):127-34.
PMID: 19959964

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