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Goat Anti-CD14 Antibody (EB07103)

Code Name Applications Availability Size Tested species Price Grade  
EB07103 Goat Anti-CD14 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $315.00 Elite

Ordering Information for

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$49.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB07103 1
EB07103 (1µg/ml) staining of PBL lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
EB07103 2
EB07103(4µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Data obtained from previous batch.
Click here to download PDF datasheet.

Target Protein

Principal Names: CD14, CD14 antigen, HGNC:1628
Official Symbol: CD14
Accession Number(s): NP_000582.1
Human GeneID(s): 929

Immunogen

Peptide with sequence C-KRVDADADPRQYAD, from the internal region of the protein sequence according to NP_000582.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 55kDa band observed in Human Lymph Node lysates (calculated MW of 40.1kDa according to NP_000582.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Funda et al, Infect Immun. 2001 Jun;69(6):3772-81; PMID: 11349042). Recommended concentration: 1-3µg/ml.
IHC: In paraffin embedded Human Spleen shows strong clustered staining in some splenocytes. Recommended concentration: 4-6µg/ml. Data obtained from previous batch.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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