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Code Name Applications Availability Size Tested species Price Grade  
EB06621 Goat Anti-CD274 / PD-L1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $315.00 Elite

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EB06621 1
EB06621 (0.1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06621 2
EB06621 (2µg/ml) staining of paraffin embedded Human Placenta. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
EB06621 3
EB06621 Negative Control showing staining of paraffin embedded Human Placenta, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: CD274 antigen, PD-L1, PDCD1LG1, B7-H, B7H1, PDL1, PDCD1L1, programmed cell death 1 ligand 1, PDL1, HGNC:17635, CD274, CD274 molecule, MGC142294, MGC142296
Official Symbol: CD274
Accession Number(s): NP_054862.1; NP_001254635.1
Human GeneID(s): 29126
Important Comments: This antibody is expected to recognize reported isoforms a and b (NP_054862.1; NP_001254635.1) only.

Immunogen

Peptide with sequence CKKQSDTHLEET, from the C Terminus of the protein sequence according to NP_054862.1; NP_001254635.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 40kDa band observed in Human Heart lysates (calculated MW of 33.3kDa according to NP_054862.1). The observed molecular weight corresponds to the glycosylated forms. Recommended concentration: 0.1-0.3µg/ml.
IHC: In paraffin embedded Human Placenta shows membranous staining of cytotrophoblasts. Recommended concentration: 2-4µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Specific References

This antibody has been successfully used in Western blot in Human:
Guozhi Xia, Xiaopu Zheng, Xinye Yao, Xiaowei Yao, Zhongwei Liu, Junkui Wang.
Expression of programmed cell death-1 and its ligand B7 homolog 1 in peripheral blood lymphocytes from patients with peripartum cardiomyopathy.
Clin Cardiol. 2016 Dec 27.
PMID: 28026044

This antibody has been successfully used in Western blot and IHC in Human:
Chen J, Li G, Meng H, Fan Y, Song Y, Wang S, Zhu F, Guo C, Zhang L, Shi Y.
Upregulation of B7-H1 expression is associated with macrophage infiltration in hepatocellular carcinomas.
Cancer Immunol Immunother. 2012 Jan;61(1):101-8.
PMID: 21853301

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