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Goat Anti-CD34-T Antibody (EB11681)

Code Name Applications Availability Size Tested species Price Grade  
EB11681 Goat Anti-CD34-T Antibody Pep-ELISA, WB, FC Yes 100µg specific antibody in 200µl Human $315.00 Elite

Ordering Information for

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB11681 1
EB11681 (2µg/ml) staining of Peripheral Blood Lymphocytes lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB11681 2
Flow Cytometry analysis of paraformaldehyde fixed Jurkat using EB11681 at 10ug/ml in green and negative control goat IgG at 0.1mg/ml in blue. Detected by Alexa Fluor®488
Click here to download PDF datasheet.

Target Protein

Principal Names: CD34, CD34 antigen, CD34 molecule, CD34-T, hematopoietic progenitor cell antigen CD34, OTTHUMP00000034733, OTTHUMP00000034734
Official Symbol: CD34
Accession Number(s): NP_001764.1
Human GeneID(s): 947
Non-Human GeneID(s): 12490 (mouse)
Important Comments: This antibody is expected to recognize reported isoform CD34-T (NP_001020280.1) only.

Immunogen

Peptide with sequence C-SPTGERLELEP, from the C-Terminus of the protein sequence according to NP_001764.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 85+95kDa bands observed in Human Peripheral Blood Mononucleocytes lysates (calculated MW of 35.0kDa according to NP_001764.1). The observed MW correspond to glycosylated forms. Recommended concentration: 1-3µg/ml.

Flow Cytometry: This antibody was used successfully on Jurkat, but from a different lot than the Western blot confirmation. Please indicate in your order the application required (ask for P1).

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse

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