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Code Name Applications Availability Size Tested species Price Grade  
EB07094 Goat Anti-CDKN2A (isoform 1) / p16INK4a Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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EB07094 1
EB07094 (1µg/ml) staining of HeLa Lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB07094 2
EB07094 (5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CDKN2A, p16INK4a, cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4), HGNC:1787, ARF, CDK4I, CDKN2, CMM2, INK4, INK4a, MLM, MTS1, TP16, p14, p14ARF, p16, p16INK4, p19, CDK4 inhibitor p16-INK4, cell cycle negative regulator beta, cyclin-
Official Symbol: CDKN2A
Accession Number(s): NP_000068.1; NP_001182061.1
Human GeneID(s): 1029
Important Comments: This antibody is expected to recognise isoform p16INK4a (NP_000068.1) and isoform p16gamma (NP_001182061.1).

Immunogen

Peptide with sequence C-HARIDAAEGPSDIPD, from the C Terminus of the protein sequence according to NP_000068.1; NP_001182061.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 16-18kDa band observed in lysates of the cell lines A431 and HeLa (calculated MW of 16.5kDa according to NP_000068.1). Recommended concentration: 0.1-1µg/ml.
IHC: Paraffin embedded Human Liver. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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