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Code Name Applications Availability Size Tested species Price Grade  
EB07092 Goat Anti-CDKN2A (isoform 3) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $315.00 Elite

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EB07092 1
EB07091 (1µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: CDKN2A, cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4), HGNC:1787, ARF, CDK4I, CDKN2, CMM2, INK4, INK4a, MLM, MTS1, TP16, p14, p14ARF, p16, p16INK4, p16INK4a, p19, CDK4 inhibitor p16-INK4, cell cycle negative regulator beta, cyclin-dependent kinase inhibitor 2A, cyclin-dependent kinase inhibitor 2A, p14ARF, cyclin-dependent kinase inhibitor p12, p16INK4a alternatively spliced form, cyclin-dependent kinase inhibitor p16, multiple tumor suppressor 1
Official Symbol: CDKN2A
Accession Number(s): NP_478104.1
Human GeneID(s): 1029
Important Comments: This antibody is expected to recognize the pancreas isoform (NP_478104.1) only.

Immunogen

Peptide with sequence C-ILRKKGRLPGEFSE, from the internal region of the protein sequence according to NP_478104.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 17kDa band observed in lysates of cell line HeLa (calculated MW of 12.1kDa according to NP_478104.1). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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