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Goat Anti-CENTA1 Antibody (EB06120)

Code Name Applications Availability Size Species reactivity Price Grade  
EB06120 Goat Anti-CENTA1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Pig, Dog $315.00 Elite

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EB06120 1
EB06120 (1µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ArfGAP with dual PH domains 1, centaurin-alpha, p42IP4, GCS1L, centaurin, alpha 1, CENTA1, ADAP1
Official Symbol: ADAP1
Accession Number(s): NP_006860.1
Human GeneID(s): 11033
Non-Human GeneID(s): 231821 (mouse), 171097 (rat)

Immunogen

Peptide with sequence C-QEYAVEAHFKHKP, from the C Terminus of the protein sequence according to NP_006860.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 40kDa band observed in Human Brain and Mouse Brain lysates (calculated MW of 43.4kDa according to NP_006860). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Pig, Dog

Specific Reference

The goat polyclonal antibody used in this paper was manufactured by us:
Moore CD, Thacker EE, Larimore J, Gaston D, Underwood A, Kearns B, Patterson SI, Jackson T, Chapleau C, Pozzo-Miller L, Theibert A.
The neuronal Arf GAP centaurin alpha1 modulates dendritic differentiation.
J Cell Sci. 2007 Aug 1;120(Pt 15):2683-93.
PMID: 17635995

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