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Goat Anti-CENTB2 / ACAP2 Antibody (EB06123)

Related Products: EBP06123 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB06123 Goat Anti-CENTB2 / ACAP2 Antibody ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog $300.00 Elite

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Now shipping direct to US customers! All orders will be shipped to you by our logistics partner in Kentucky. Almost all products are held in stock for immediate shipment, see individual product pages for availability. Orders are placed with and invoiced by Everest Biotech Ltd in the United Kingdom.

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Axxora, LLC

6181 Cornerstone Court East Suite 103,
San Diego
CA 92121-4727
USA

Tel: 858-550-8830
Toll Free Tel: 1-800-550-3033
Fax: 858-550-8825
Toll Free Fax: 1-800-550-8825
Email: axxora-usa@axxora.com
Website: www.axxora.com
EB06123 1
EB06123 (1µg/ml) staining of MOLT4 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06123 2
EB06123 (5µg/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ArfGAP with coiled-coil, ankyrin repeat and PH domains 2, Arf GAP with coiled coil, ANK repeat and PH domains 2, centaurin beta2, KIAA0041, CNT-B2, centaurin, beta 2, CENTB2, ACAP2
Official Symbol: ACAP2
Accession Number(s): NP_036419.3
Human GeneID(s): 23527
Non-Human GeneID(s): 78618 (mouse)

Immunogen

Peptide with sequence C-EKLNRFQQDSQKF, from the C Terminus of the protein sequence according to NP_036419.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 90kDa band observed in Human T cell line MOLT4 lysates (calculated MW of 88.1kDa according to NP_036419). Recommended concentration: 1-3µg/ml.
IHC: In paraffin embedded Human Lung shows strong staining of Type II pneumocytes. Recommended concentration, 5-10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

Background Reference

Jackson TR, Brown FD, Nie Z, Miura K, Foroni L, Sun J, Hsu VW, Donaldson JG, Randazzo PA.
ACAPs are arf6 GTPase-activating proteins that function in the cell periphery.
J Cell Biol. 2000 Oct 30;151(3):627-38.
PMID: 11062263

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