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Goat Anti-CGR19 / CGRRF1 Antibody (EB05818)

Code Name Applications Availability Size Species reactivity Price Grade  
EB05818 Goat Anti-CGR19 / CGRRF1 Antibody Pep-ELISA Yes 100µg specific antibody in 200µl Human $210.00 Aspiring

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Target Protein

Principal Names: CGRRF1, cell growth regulator with ring finger domain 1, RNF197, cell growth regulator with ring finger domain 1, CGR19, CGRRF1
Official Symbol: CGRRF1
Accession Number(s): NP_006559.1
Human GeneID(s): 10668

Immunogen

Peptide with sequence CSQKEQDKDKPKTL, from the C Terminus of the protein sequence according to NP_006559.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Preliminary experiments gave approx 60kDa and 45kDa bands in H460 lysates at 0.5ug/ml. Please note that currently we cannot find an explanation in the literature for the bands we observe given the predicted size of approx. 38kDda according to NP_006559. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested:
Expected from sequence similarity: Human

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