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Goat Anti-CHFR Antibody (EB05819)
| Code | Name | Applications | Availability | Size | Species reactivity | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB05819 | Goat Anti-CHFR Antibody | Pep-ELISA, IHC |  |
100µg specific antibody in 200µl | Human, Mouse, Rat, Pig Cow | $315.00 |  |
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Ordering Information forOrder direct from Everest
$40.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be processed and invoiced by our US partner Abcore LLC. Order offlineNow shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability. Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:
In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day. Orders are placed with and invoiced by our US partner Abcore LLC.
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Target Protein
Principal Names: RING finger protein 196, E3 ubiquitin-protein ligase CHFR, RNF196, RNF116, FLJ33629, FLJ10796, checkpoint with forkhead and ring finger domains, CHFROfficial Symbol: CHFR
Accession Number(s): NP_001154816.1; NP_001154817.1; NP_001154818.1; NP_060693.2; NP_001154819.1
Human GeneID(s): 55743
Non-Human GeneID(s): 231600 (mouse), 288734 (rat)
Important Comments: This antibody is expected to recognise all reported isoforms.
Immunogen
Peptide with sequence KFNHICEQTRFKN, from the C Terminus of the protein sequence according to NP_001154816.1; NP_001154817.1; NP_001154818.1; NP_060693.2; NP_001154819.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:64000.Western blot: Preliminary experiments gave an approx 80kDa band in human brain lysates at 0.5ug/ml, this band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 72kDda according to NP_060693. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
IHC: In paraffin embedded human brain (cerebellum) shows stained Purkinje cells. Recommended concentration, 2.5µg/ml.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human, Mouse, Rat, Pig Cow