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Goat Anti-CHMP5 Antibody (EB06716)

Code Name Applications Availability Size Species reactivity Price Grade  
EB06716 Goat Anti-CHMP5 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Cow $315.00 Elite

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EB06716 1
EB06716 (xµg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: Vps60, chromosome 9 open reading frame 83, SNF7 domain containing 2, SNF7DC2, PNAS-2, HSPC177, CGI-34, C9orf83, HGNC:26942, chromatin modifying protein 5, CHMP5
Official Symbol: CHMP5
Accession Number(s): NP_057494.3
Human GeneID(s): 51510

Immunogen

Peptide with sequence C-NKDGVLVDEFGLPQ, from the C Terminus of the protein sequence according to NP_057494.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 28kDa band observed in lysates of K562 (calculated MW of 24.5kDa according to NP_057494.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ward et al, J Biol Chem. 2005 Mar 18;280(11): 10548-55.; PMID: 15644320). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Cow

Specific Reference

The goat polyclonal antibody used in this paper was manufactured by us:
Yang D, Rismanchi N, Renvoisé B, Lippincott-Schwartz J, Blackstone C, Hurley JH.
Structural basis for midbody targeting of spastin by the ESCRT-III protein CHMP1B.
Nat Struct Mol Biol. 2008 Dec;15(12):1278-86.
PMID: 18997780

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