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Goat Anti-CLIM1 / PDLIM1 Antibody (EB06229)

Code Name Applications Availability Size Species reactivity Price Grade  
EB06229 Goat Anti-CLIM1 / PDLIM1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $315.00 Elite

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Orders are placed with and invoiced by our US partner Abcore LLC.

 

EB06229 1
EB06229 staining (0.1µg/ml) of human lung lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: enigma, elfin, PDZ and LIM domain 1, carboxy terminal LIM domain protein 1, PDZ and LIM domain 1 (elfin), hCLIM1, CLP-36, ELFIN, CLP36, CLIM1, PDLIM1
Official Symbol: PDLIM1
Accession Number(s): NP_066272.1
Human GeneID(s): 9124

Immunogen

Peptide with sequence C-TPPEGYEVVTVFPK, from the C Terminus of the protein sequence according to NP_066272.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 37kDa band observed in human lung and heart lysates (calculated MW of 36.1kDa according to NP_066272.1). Recommended for use at 0.1-0.3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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