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Goat Anti-Clusterin / APOJ Antibody (EB06929)

Related Products: EBP06929 - Immunizing Peptide, EBP11646 - Immunizing Peptide, Goat Anti-Clusterin / APOJ (aa44-58) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB06929 Goat Anti-Clusterin / APOJ Antibody Pep-ELISA, WB, IF, IHC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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EB06929 1
EB06929 (0.1µg/ml) staining of Human Colon lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06929 2
EB06929 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing ER/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06929 3
EB06929 (2.5µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Target Protein

Principal Names: APOJ, clusterin, CLU, complement lysis inhibitor, SP-40,40, sulfated glycoprotein 2, testosterone-repressed prostate message 2, apolipoprotein J, HGNC:2095, CLI, MGC24903, SGP-2, SGP2, SP-40, TRPM-2, TRPM2, apolipoprotein J, clusterin, complement-associated protein SP-40, sulfated glycoprotein 2, AAG4, KUB1, aging-associated protein 4, clusterin (complement lysis inhibitor, SP-40,40, sulfated glycoprotein 2, testosterone-repressed prostate message 2, apolipoprotein J)
Official Symbol: CLU
Accession Number(s): NP_001822.2; NP_976084.1
Human GeneID(s): 1191
Important Comments: This antibody is expected to recognise both reported isoforms (NP_001822.2 and NP_976084.1)

Immunogen

Peptide with sequence C-EKALQEYRKKHREE, from the C Terminus of the protein sequence according to NP_001822.2; NP_976084.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 35-37kDa band observed in Human Colon and Prostate lysates (calculated MW of 57.8kDa according to NP_NP_001822.2 and 52.5 according to NP_976084.1). The observed molecular weight corresponds to earlier findings in literature (PMID: 9713325 and PMID: 11560857). Recommended concentration: 0.03-0.1µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Tonsil. Recommended concentration: 2.5µg/ml.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10µg/ml

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog

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FAQs

In which tissues / cell lines has this antibody been tested?

This antibody has been tested in human colon, human kidney and human prostate lysates.