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Goat Anti-COMT (internal) Antibody (EB07357)

Related Products: EBP06595 - Immunizing Peptide, EBP07357 - Immunizing Peptide, Goat Anti-COMT (N Terminus) Antibody
Code Name Applications Availability Size Species reactivity Price Grade  
EB07357 Goat Anti-COMT (internal) Antibody Pep-ELISA, WB, EIA Yes 100µg specific antibody in 200µl Human $315.00 Elite

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EB07357 1
Staining of human testis lysate (35µg protein in RIPA buffer) with EB07357 (0.03µg/ml). Primary incubation was 1 hour. Detected by chemiluminescence.
EB07357 2
EB7357 (0.5ug/ml) as the reporter with EB002002 as the capture rabbit antibody (2.5ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: catechol-O-methyltransferase, COMT
Official Symbol: COMT
Accession Number(s): NP_000745.1; NP_009294.1
Human GeneID(s): 1312
Important Comments: This antibody is expected to recognise both reported isoforms (NP_000745.1 and NP_009294.1)

Immunogen

Peptide with sequence C-QDIIPQLKKKYDVD, from the internal region of the protein sequence according to NP_000745.1; NP_009294.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 28kDa band and faint 25kDa band observed in human testis lysates (calculated MW of 30.0kDa according to NP_000745.1 and 24.5kDa according to NP_009294.1). Recommended concentration: 0.03-0.1µg/ml.

Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant COMTcaptured by a rabbit antibody. Recommended reporter concentration: 0.5-2ug/ml

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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