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Goat Anti-COX1 / PTGS1 Antibody (EB05285)

Code Name Applications Availability Size Tested species Price Grade  
EB05285 Goat Anti-COX1 / PTGS1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $315.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB05285 1
EB05285 (2µg/ml) staining of U937 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB05285 2
EB05285 (0.5µg/ml) staining of Mouse Kidney (A) and Skeletal Muscle (B) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB05285 3
EB05285 (3.8µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
Click here to download PDF datasheet.

Target Protein

Principal Names: PTGS1, COX1, COX3, PHS1, PCOX1, PGHS1, PTGHS, PGG/HS, PGHS-1, prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase), prostaglandin G/H synthase and cyclooxygenase, RP11-542K23.6
Official Symbol: PTGS1
Accession Number(s): NP_000953.2; NP_542158.1
Human GeneID(s): 5742
Important Comments: This antibody is expected to recognise both human isoforms of this protein according to NP_000953.2 and NP_542158.1.

Immunogen

Peptide with sequence C-QDDGPAVERPSTEL, from the C Terminus of the protein sequence according to NP_000953.2; NP_542158.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 60kDa and 70-75kDa bands observed in lysates of cell line U937 and approx 70-75kDa band observed in Mouse Kidney and Skeletal Muscle lysates (calculated MW of 64.5kDa according to Human NP_542158.1 and 68.7kDa Human NP_000953.2, and 69.0kDa according to Mouse NP_032995.1). The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-2µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human

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