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Goat Anti-COX4I1, Biotinylated Antibody (EB07914-B)

Code Name Applications Availability Size Tested species Price Grade  
EB07914-B Goat Anti-COX4I1, Biotinylated Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB07914-B 1
Biotinylated EB07914 (0.1µg/ml) staining of Human Skeletal Muscle lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
Click here to download PDF datasheet.

Target Protein

Principal Names: COX4I1, cytochrome c oxidase subunit IV isoform 1, COX4, COX4-1, COXIV, COXIV-1, cytochrome c oxidase polypeptide IV
Official Symbol: COX4I1
Accession Number(s): NP_001852.1; NP_001305726.1
Human GeneID(s): 1327
Important Comments: This antibody is expected to recognize reported isoforms 1 and 4 (NP_001852.1; NP_001305726.1). Reported variants represent identical protein: NP_001305715.1, NP_001852.1. This product may cross-react to COX4I2 (GeneID 84701).

Immunogen

Peptide with sequence C-QGLASKWDYEKNE., from the C Terminus of the protein sequence according to NP_001852.1; NP_001305726.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 17kDa band observed in Human Skeletal Muscle lysates (calculated MW of 19.6kDa according to NP_001852.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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