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Goat Anti-CRHR1 / CRF-R (aa 107 - 117) Antibody (EB08035)

Code Name Applications Availability Size Tested species Price Grade  
EB08035 Goat Anti-CRHR1 / CRF-R (aa 107 - 117) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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EB08035 1
EB08035 (1µg/ml) staining of Human Colon (A), Cerebellum (B), Rat Brain (C) and Mouse spinal Cord (D) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB08035 2
Confocal image (3µm) of EB08035 (0.3µg/ml) staining in green a dendrite of a neuronal cell in C57 mouse cortex. Counter-stained with DAPI (in blue). Data kindly provided by Dr Y. Chen, TZ Baram lab, UC Irvine, CA. This data is from a previous batch.
Click here to download PDF datasheet.

Target Protein

Principal Names: CRHR1, CRF-R, corticotropin releasing hormone receptor 1, CRF1, CRFR1, CRH-R1h, CRHR, CRHR1f, corticotropin releasing hormone receptor variant 1h, seven transmembrane helix receptor
Official Symbol: CRHR1
Accession Number(s): NP_004373.2
Human GeneID(s): 1394
Non-Human GeneID(s): 12921 (mouse), 58959 (rat)

Immunogen

Peptide with sequence C-NEEKKSKVHYH, from the internal region of the protein sequence according to NP_004373.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 60kDa band observed in Human Brain (Cerebellum), Colon, Rat Brain and Mouse spinal cord lysates (calculated MW of 47.7kDa according to NP_004373.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Xu et al, Mol Pharmacol. 2001 Mar;59(3):485-92; PMID: 11179443) . Recommended concentration: 0.1-0.3µg/ml.

Immunocytochemistry: This data if from a previous batch: http://www.biocompare.com/Product-Reviews/152450-Immunocytochemistry-for-CRH-receptor-type-1/

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat

Specific References

This antibody has been successfully used in IHC in Mouse:
Jüngling K, Liu X, Lesting J, Coulon P, Sosulina L, Reinscheid RK, Pape HC.
Activation of neuropeptide S expressing neurons in the locus coeruleus by corticotropin-releasing factor.
J Physiol. 2012 May 8.
PMID: 22570383

This antibody has been successfully used in Western blot in Mouse:
Weerachayaphorn J, Mennone A, Soroka CJ, Harry K, Hagey LR, Kensler TW, Boyer JL.
Nuclear factor-E2-related factor 2 is a major determinant of bile acid homeostasis in the liver and intestine.
Am J Physiol Gastrointest Liver Physiol. 2012 May 1;302(9):G925-36.
PMID: 22345550

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