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Goat Anti-CRHR1 / CRF-R (aa 250 - 263) Antibody (EB07553)

Code Name Applications Availability Size Tested species Price Grade  
EB07553 Goat Anti-CRHR1 / CRF-R (aa 250 - 263) Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $315.00 Elite

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ARP American Research Products, Inc
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EB07553 1
EB07553 (5µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CRHR1, CRF-R, corticotropin releasing hormone receptor 1, CRF1, CRFR1, CRH-R1h, CRHR, CRHR1f, corticotropin releasing hormone receptor variant 1h, seven transmembrane helix receptor
Official Symbol: CRHR1
Accession Number(s): NP_004373.2
Human GeneID(s): 1394
Non-Human GeneID(s): 12921 (mouse), 58959 (rat)


Peptide with sequence KLYYDNEKCWFGKR, from the internal region of the protein sequence according to NP_004373.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Sheep

Specific References

This antibody (previous batch) has been successfully used in IF on Mouse:
Jiang Z, Rajamanickam S, Justice NJ.
Local Corticotropin-Releasing Factor Signaling in the Hypothalamic Paraventricular Nucleus.
J Neurosci. 2018 Feb 21;38(8):1874-1890.
PMID: 29352046

This antibody (previous batch) has been successfully used in ICC on Rat:
Adrienne L. Andres, Limor Regev, Lucas Phi, Ronald R. Seese, Yuncai Chen, Christine M. Gall and Tallie Z. Baram
NMDA Receptor Activation and Calpain Contribute to Disruption of Dendritic Spines by the Stress Neuropeptide CRH
J Neurosci. 2013 Oct 23;33(43):16945-60.
PMID: 24155300

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