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Goat Anti-cytochrome P450 1A1 Antibody (EB11172)

Code Name Applications Availability Size Tested species Price Grade  
EB11172 Goat Anti-cytochrome P450 1A1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
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Email: info@arp1.com
www.arp1.com

EB11172 1
EB11172 (0.3µg/ml) staining of A549 (A) and MCF7 (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: AHH, AHRR, aryl hydrocarbon hydroxylase, CP11, CYP1, CYPIA1, cytochrome P1-450, dioxin-inducible, cytochrome P450 1A1, cytochrome P450 form 6, cytochrome P450, family 1, subfamily A, polypeptide 1, cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 1, cytochrome P450-C, cytochrome P450-P1, flavoprotein-linked monooxygenase, OTTHUMP00000183469, P1-450, P450-C, P450DX, xenobiotic monooxygenase, CYP1A1
Official Symbol: CYP1A1
Accession Number(s): NP_000490.1
Human GeneID(s): 1543
Important Comments: This antibody is not expected to cross-react with the similar P450 1A2

Immunogen

Peptide with sequence CQEKQLDENANVQ, from the internal region of the protein sequence according to NP_000490.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 85-90kDa band observed in lysates of cell lines A549 and MCF7 (calculated MW of 58.2kDa according to NP_000490.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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