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Goat Anti-DCP1A Antibody (EB07125)

Code Name Applications Availability Size Species reactivity Price Grade  
EB07125 Goat Anti-DCP1A Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $315.00 Elite

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EB07125 1
EB07125 (2µg/ml) staining of A431 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: transcription factor SMIF, putative protein product of Nbla00360, decapping enzyme hDcp1a, Smad4-interacting transcriptional co-activator, SMIF, SMAD4IP1, Nbla00360, HSA275986, decapping enzyme, DCP1A
Official Symbol: DCP1A
Accession Number(s): NP_060873.3
Human GeneID(s): 55802
Non-Human GeneID(s): 75901 (mouse)

Immunogen

Peptide with sequence C-QVLTKNKDNHN, from the C Terminus of the protein sequence according to NP_060873.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 75kDa band observed in human lysates of A431 and HeLa (calculated MW of 63.3kDa according to NP_060873.3). Recommended concentration: 1-3µg/ml. An additional band of 48kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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